Cohnella luojiensis sp. nov., isolated from soil of a Euphrates poplar forest.

A novel strain, HY-22R(T), was isolated from soil of a Euphrates poplar forest in Xinjiang, China. The cells were Gram-positive-staining, rod-shaped and motile by means of peritrichous flagella. Growth occurred at 10-37 degrees C (optimum 30 degrees C), at pH 7.0-8.0 (optimum pH 7.0) and with 0-1 % NaCl. A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HY-22R(T) was closely related to Cohnella phaseoli GSPC1(T) (96.3 % sequence similarity). The major respiratory quinone was MK-7 and the predominant fatty acids were anteiso-C(15 : 0), iso-C(15 : 0), iso-C(16 : 0) and C(16 : 0). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 49.6 mol%. On the basis of the phylogenetic, physiological and chemotaxonomic data, strain HY-22R(T) represents a novel species in the genus Cohnella, for which the name Cohnella luojiensis sp. nov. is proposed. The type strain is HY-22R(T) (=CCTCC AB 208254(T) =NRRL B-59213(T)).